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New England Biolabs onetaq quick load 2x master mix
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separation gel  (Bio-Rad)
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Fig. 4. <t>Separation</t> of European eel seminal plasma (1 μg of protein/μl, n = 9 sperm samples) by one-dimensional SDS-PAGE (15% acrylamide). The columns 2–5 and 6–10 are different sperm samples in 6th and 13th week of hormonal treatment respectively. Asterisks indicate significant differences between 19 and 90 kDa band. Proteins were stained with Coomassie Brilliant Blue R-240.
Separation Gel, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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loading buffer  (Bio-Rad)
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Fig. 4. <t>Separation</t> of European eel seminal plasma (1 μg of protein/μl, n = 9 sperm samples) by one-dimensional SDS-PAGE (15% acrylamide). The columns 2–5 and 6–10 are different sperm samples in 6th and 13th week of hormonal treatment respectively. Asterisks indicate significant differences between 19 and 90 kDa band. Proteins were stained with Coomassie Brilliant Blue R-240.
Loading Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 4. <t>Separation</t> of European eel seminal plasma (1 μg of protein/μl, n = 9 sperm samples) by one-dimensional SDS-PAGE (15% acrylamide). The columns 2–5 and 6–10 are different sperm samples in 6th and 13th week of hormonal treatment respectively. Asterisks indicate significant differences between 19 and 90 kDa band. Proteins were stained with Coomassie Brilliant Blue R-240.
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scanning ocular fluorophotometer  (Ocumetrics Inc)
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Fig. 4. <t>Separation</t> of European eel seminal plasma (1 μg of protein/μl, n = 9 sperm samples) by one-dimensional SDS-PAGE (15% acrylamide). The columns 2–5 and 6–10 are different sperm samples in 6th and 13th week of hormonal treatment respectively. Asterisks indicate significant differences between 19 and 90 kDa band. Proteins were stained with Coomassie Brilliant Blue R-240.
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custom designed macro in powerlab 8/30 software  (POWERLAB INC)
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Fig. 4. <t>Separation</t> of European eel seminal plasma (1 μg of protein/μl, n = 9 sperm samples) by one-dimensional SDS-PAGE (15% acrylamide). The columns 2–5 and 6–10 are different sperm samples in 6th and 13th week of hormonal treatment respectively. Asterisks indicate significant differences between 19 and 90 kDa band. Proteins were stained with Coomassie Brilliant Blue R-240.
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chi squared  (CH Instruments)
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CH Instruments chi squared
Fig. 4. <t>Separation</t> of European eel seminal plasma (1 μg of protein/μl, n = 9 sperm samples) by one-dimensional SDS-PAGE (15% acrylamide). The columns 2–5 and 6–10 are different sperm samples in 6th and 13th week of hormonal treatment respectively. Asterisks indicate significant differences between 19 and 90 kDa band. Proteins were stained with Coomassie Brilliant Blue R-240.
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lubac inhibitor hoipin-8  (Axon Medchem LLC)
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Fig. 4. <t>Separation</t> of European eel seminal plasma (1 μg of protein/μl, n = 9 sperm samples) by one-dimensional SDS-PAGE (15% acrylamide). The columns 2–5 and 6–10 are different sperm samples in 6th and 13th week of hormonal treatment respectively. Asterisks indicate significant differences between 19 and 90 kDa band. Proteins were stained with Coomassie Brilliant Blue R-240.
Lubac Inhibitor Hoipin 8, supplied by Axon Medchem LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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quad bridge amp  (POWERLAB INC)
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Fig. 4. <t>Separation</t> of European eel seminal plasma (1 μg of protein/μl, n = 9 sperm samples) by one-dimensional SDS-PAGE (15% acrylamide). The columns 2–5 and 6–10 are different sperm samples in 6th and 13th week of hormonal treatment respectively. Asterisks indicate significant differences between 19 and 90 kDa band. Proteins were stained with Coomassie Brilliant Blue R-240.
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Fig. 4. Separation of European eel seminal plasma (1 μg of protein/μl, n = 9 sperm samples) by one-dimensional SDS-PAGE (15% acrylamide). The columns 2–5 and 6–10 are different sperm samples in 6th and 13th week of hormonal treatment respectively. Asterisks indicate significant differences between 19 and 90 kDa band. Proteins were stained with Coomassie Brilliant Blue R-240.

Journal: Comparative biochemistry and physiology. Part A, Molecular & integrative physiology

Article Title: Identification of the major proteins present in the seminal plasma of European eel, and how hormonal treatment affects their evolution. Correlation with sperm quality.

doi: 10.1016/j.cbpa.2016.06.025

Figure Lengend Snippet: Fig. 4. Separation of European eel seminal plasma (1 μg of protein/μl, n = 9 sperm samples) by one-dimensional SDS-PAGE (15% acrylamide). The columns 2–5 and 6–10 are different sperm samples in 6th and 13th week of hormonal treatment respectively. Asterisks indicate significant differences between 19 and 90 kDa band. Proteins were stained with Coomassie Brilliant Blue R-240.

Article Snippet: IPG stripswere placed onto seconddimension SDS-PAGE (overall gel size 18.3 × 20.0 × 0.1 cm) which was performed using 1.5 cm 4% stacking gel (0.5 M Tris–HCl pH 8.8, 30% acrylamide, 10% SDS, 10% APS, 0.1% TEMED) and 15% separation gel (1.5M Tris–HCl pH 8.8, 30% acrylamide, 10% SDS, 10% APS, 0.05% TEMED) using a Protean IIxi device (BioRad, Hercules, CA, USA).

Techniques: Clinical Proteomics, SDS Page, Staining